LT2ESWTR Source Water Monitoring for Systems Serving At Least 10,000 People Factsheet

WHAT IS THE LT2ESWTR?

The U.S. Environmental Protection Agency (EPA) published the Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR) on January 5, 2006. The LT2ESWTR improves control of microbial pathogens. The LT2ESWTR requires source water monitoring at public water systems (PWSs) that use surface water or ground water under the direct influence of surface water (GWUDI) (i.e., Subpart H PWSs). Based on system size and filtration type, systems need to monitor for Cryptosporidium, E. coli, and turbidity.

WHAT IS THE PURPOSE OF SOURCE WATER MONITORING?

Source water monitoring data will be used to categorize the source water Cryptosporidium concentration into one of four “bin” classifications that have associated treatment requirements. The LT2ESWTR provides other options for systems to comply with the initial source water monitoring requirements:

Submit data from Cryptosporidium samples collected before the system must begin source water monitoring (i.e., Grandfathered), and the data must meets certain requirements.

Filtered systems may skip source water monitoring and commit to provide a total of at least 5.5- log of treatment for Cryptosporidium, equivalent to meeting the treatment requirement of Bin 4. Unfiltered systems skip source water monitoring and commit to provide a total of at least 3-log Cryptosporidium inactivation, which is equal to meeting the treatment requirements for unfiltered systems with a mean Cryptosporidium concentration of greater than 0.01 oocysts/L. Systems that decide to skip monitoring and provide maximum treatment must notify the state in writing.

A second round of source water monitoring will follow 6 years after the system makes its initial bin determination. Grandfathering is not available for the second round of source water monitoring.

Note: E. coli and turbidity data may not be grandfathered unless the system is also grandfathering corresponding Cryptosporidium data.

WHAT ARE THE INITIAL SOURCE WATER MONITORING REQUIREMENTS?

The source water monitoring requirements of the LT2ESWTR apply to all Subpart H PWSs. You are subject to initial source water monitoring requirements if you do not have existing monitoring data that meets grandfathering requirements. For more information on source water monitoring requirements, see EPA’s Source Water Monitoring Guidance Manual for Public Water Systems for the Final Long Term 2 Enhanced Surface Water Treatment Rule (EPA 815-R06-005 February 2006), available at www.epa.gov/safewater/disinfection/lt2/compliance.html.

Prior to beginning initial source water monitoring, you must submit a sampling schedule that specifies the calendar dates when you will collect the required source water samples. The samples must be evenly spaced throughout the monitoring period (e.g., monthly on the 15th of each month). However, the schedule may be altered to take into account holidays, weekends, or other events. All the samples must be taken within a 5-day window (i.e., you can take the sample up to 2 days before or 2 days after

the date indicated in the schedule). In addition, you must submit a description of the intended sampling location in relation to the source and any treatment processes, as well as a description of any points of chemical addition, and filter backwash recycle.

FILTERED SYSTEMS SERVING AT LEAST 10,000 PEOPLE – You must collect Cryptosporidium,

coli and turbidity samples at least monthly for 24 months.

UNFILTERED SYSTEMS SERVING AT LEAST 10,000 PEOPLE – You must sample for Cryptosporidium

at least monthly for 24 months.

Alternately, you may notify the EPA or the state that you elect not to conduct source water monitoring and commit to providing the maximum treatment of 5.5 log removal or inactivation for filtered systems or 3-log inactivation for unfiltered systems.

WHEN MUST I COMPLY WITH THE MONITORING REQUIREMENTS?

The system compliance schedule is based on the population served by your system. A PWS must conduct monitoring based on the requirements of the largest system in the combined distribution system. The interconnected wholesale/consecutive systems relationships have been determined by the state.

Systems that serve…	> 100,000 people (Schedule 1) 1	50,000 to 99,999 people (Schedule 2) 1	10,000 to 49,999 people (Schedule 3) 1
Submit: Sample Schedule and Sample Location Description	July 1, 2006	January 1, 2007	January 1, 2008
Must begin the first round of source water monitoring by…	October 2006	April 2007	April 2008
Submit Grandfathered Data (if applicable)	December 1, 2006	June 1, 2007	June 1, 2008
Submit Bin Classification (Filtered) or Mean Cryptosporidium Level (Unfiltered)	March 2009	September 2009	September 2010
Comply with additional LT2ESWTR treatment technique requirements2	April 1, 2012	October 1, 2012	October 1, 2013
Must begin the second round of source water monitoring by…	April 2015	October 2015	October 2016

1 Your schedule is defined by the largest system in your combined distribution system.

2 State may allow up to an additional 2 years for capital improvements to comply with the treatment technique.

WHAT IS A BIN CLASSIFICATION?

FILTERED SYSTEMS SERVING AT LEAST 10,000 PEOPLE – You will be classified into a “bin” based on the results of your source water monitoring. Your bin classification determines whether further treatment for Cryptosporidium is required. A second round of source water monitoring is required 6 years after your initial bin classification and may affect your bin classification.

For systems that are:	Mean Cryptosporidium Concentration1	Bin Classification
…required to monitor for Cryptosporidium	< 0.075 oocysts/L	Bin 1
	from 0.075 to < 1.0 oocysts/L	Bin 2
	from 1.0 to < 3.0 oocysts/L	Bin 3
	> 3.0 oocysts/L	Bin 4

1 Samples must be analyzed by an approved laboratory and use EPA method 1622 or 1623.

ADDITIONAL TREATMENT REQUIREMENTS FOR FILTERED SYSTEMS – Additional treatment is required if the bin classification is a 2, 3, or 4. Refer to the table below for the additional Cryptosporidium treatment requirements.

Bin Classification	If the system uses the following filtration treatment in full compliance with existing requirements, then the additional Cryptosporidium treatment requirements are…
Bin Classification	Conventional filtration treatment (including softening)	Direct filtration	Slow sand or diatomaceous earth filtration	Alternative filtration technologies
Bin 1	No additional treatment	No additional treatment	No additional treatment	No additional treatment
Bin 2	1-log treatment	1.5-log treatment	1-log treatment	(1)
Bin 3	2-log treatment	2.5-log treatment	2-log treatment	(2)
Bin 4	2.5-log treatment	3-log treatment	2.5-log treatment	(3)

As determined by the state such that the total Cryptosporidium removal and inactivation is at least 0-log.
As determined by the state such that the total Cryptosporidium removal and inactivation is at least 0-log.
As determined by the state such that the total Cryptosporidium removal and inactivation is at least 5-log.

For information on the toolbox options that can be used to achieve additional log removal requirements, see the Long Term 2 Enhanced Surface Water Treatment Rule Toolbox Guidance Manual (draft version anticipated late 2006).

UNFILTERED SYSTEMS SERVING AT LEAST 10,000 PEOPLE – You must calculate an arithmetic mean of all Cryptosporidium samples concentrations required. Following completion of the second round of source water monitoring, you must provide a level of inactivation for Cryptosporidium based on the arithmetic mean of your Cryptosporidium sample concentrations.

For systems that are:

Mean Cryptosporidium

Concentration1

Cryptosporidium inactivation

Unfiltered

< 0.01 oocysts/L

2-log

> 0.01 oocysts/L

3-log

1 Samples must be analyzed by an approved laboratory and use EPA method 1622 or 1623.

ARE YOU CONSIDERING MAKING A CHANGE TO YOUR DISINFECTION PRACTICES?

After completing the initial round of source water monitoring, systems that plan to make a significant change to their disinfection practice must notify the state, develop disinfection profiles, and calculate disinfection benchmarks for Giardia lamblia and viruses. To develop a profile and benchmark, PWSs must monitor at least weekly for a period of 12 consecutive months to determine the total log inactivation for Giardia lamblia and viruses. The disinfection benchmark is an indicator of disinfection effectiveness and depends upon the inactivation of Giardia lamblia or viruses. The benchmark is determined by calculating the average daily inactivation value for each of 12 consecutive months. The lowest monthly average becomes the disinfection benchmark. If the PWS has data from more than 1 year, the benchmark is the average of the lowest monthly average value for each of the years. A PWS may use grandfathered data that is substantially equivalent to develop the disinfection profiles for Giardia lamblia and viruses. The Long Term 1 Enhanced Surface Water Treatment Rule (LT1ESWTR) Disinfection Profiling and Benchmarking Technical Guidance Manual (EPA 816-R-03-004, May 2003), provides guidance for developing a disinfection profile and benchmark. EPA has developed two tools for systems to determine their disinfection profile and calculate the benchmark at the following website: http://www.epa.gov/safewater/mdbp/lt1eswtr.html.

ADDITIONAL GUIDANCE MATERIALS

The following guidance document addresses the source water monitoring requirements for the LT2ESWTR:

Source Water Monitoring Guidance Manual for Public Water Systems for the Final Long Term 2 Enhanced Surface Water Treatment Rule (EPA 815-R06-005 February 2006) – Provides surface water systems, laboratories, states, and Tribes with a review of the source water monitoring provisions. The source water monitoring guidance manual provides direction to the systems on how, where and when to monitor, how to report the data, how to submit “grandfathered” data (e.g., previously collected data), and how the data can be evaluated and used to determine risk bin classification.

For additional guidance on implementing the LT2ESWTR, you may refer to the following existing and future EPA materials:

LT2ESWTR Quick Reference Guides (Schedules 1 – 3) On-line Microscopy Training Module

On-line Sample Collection Module

Microbial Laboratory Guidance Manual for the Final Long Term 2 Enhanced Surface Water Treatment Rule (EPA 815-R06-006 February 2006)

Membrane Filtration Guidance Manual (EPA 815-R-06-009 November 2005)

Membrane Filtration Guidance Manual: Overview and Summary Factsheet (www.epa.gov/safewater/disinfection/lt2/pdfs/guide_lt2_membranefiltration_fs_final.pdf)

Ultraviolet Disinfection Guidance Manual and Workbook (final version anticipated mid-2006)

Simultaneous Compliance Guidance Manual for Stage 2 Rules (draft version anticipated mid-2006)

Long Term 2 Enhanced Surface Water Treatment Rule Toolbox Guidance Manual (draft version anticipated late 2006)

For additional information, please contact the Safe Drinking Water Hotline at 1-800-426-4791, send an email to stage2mdbp@epa.gov, or visit www.epa.gov/safewater/disinfection/lt2.

Office of Water (4606) EPA 816-F-06-017 www.epa.gov/safewater/disinfection/lt2 June 2006

Microscopic Particulate Analysis ( MPA ) for Ground Water Under Direct Influence ( GWUDI ) Guide

Microscopic Particulate Analysis (MPA) For Evaluation of Ground Water

I. What is “a ground water source under direct influence of surface water”?

The EPA Guidance Manual defines a ground water source under direct influence of surface water as water in which there is either:

“significant occurrence of insects or macroorganisms, algae, organic debris, or large-diameter pathogens such as Giardia lamblia.”

“significant and relatively rapid shifts in water characteristics such as turbidity, temperature, conductivity, or pH which closely correlate to climatological or surface water condition.”

II. What information is useful in classification?

Historical water quality records

At least three years of Total Coliform and/or Fecal Coliform
Turbidity and temperature records including those of nearby surface water
No history of a known or suspected outbreak of Giardia, Cryptosporidium or other pathogenic organisms associated with surface water that has been attributed to the
No evidence of particulate matter associated with surface

On site inspection

No evidence for surface water
Sufficient distances from surface water

III. What is MPA and how can it be used to differentiate ground water under surface influence?

The premise behind the use of microscopic particulate analysis (MPA) is that surface waters are subject to contamination by pathogens such as Giardia and that there are other organisms whose natural habitat is limited to surface waters. If these surface water organisms are found in ground water, then the water is subject to contamination with Giardia cysts and other pathogens. Indicators of surface water contamination of ground waters include: Giardia, coccidia including Cryptosporidium, diatoms and certain other algae, rotifers, green plant material, and insect parts.

Cryptosporidium

Cryptosporidium

Cymbella (Diatom)

Naviculla (Diatom)

Naviculla (Diatom)

IV. How is test conducted?

Samples are collected in accordance with the EPA “Consensus Method for Determining Groundwaters Under the Direct Influence of Surface Water Using Microscopic Particulate Analysis (MPA).” Two sampling events are recommended, one during a dry period and a second during a wet period. Turbidity, temperature, rainfall and stream flow records, and conductivity etc. may be used for guidance for when to test.

V. How are results interpreted?

When organisms such as Giardia , coccidian, such as Cryptosporidium, insect parts including nymphs, larvae and eggs, rotifers, diatoms and other algae are detected in groundwater, they are useful as indicators of surface contamination. The process of scoring microscopic results with relative risk factors is beneficial in classifying questionable supplies.

Primary Indicators

Giardia – A protozoan parasite. Occurrence in water sample must be confirmed by identification of two or more morphological characteristics, nuclei, axoneme and/or median body.

Coccidia (Cryptosporidium and other coccidia) – Coccidia are protozoan parasites of vertebrates. Cryptosporidium, a pathogen of concern to human health is small in size (approximately 4-7 µm diameter). Cryptosporidium is very difficult to identify without IFA staining, and its occurrence is confirmed by identification of sporozoites within the oocyst.

Diatoms – Diatoms are prevalent in creeks and streams, and they require sunlight for photosynthesis and continued survival. It is important that the diatoms contain normal internal morphology including pigment in order to be significant for the purposes of this analysis.

Other Algae – Includes several groups of algae including the green and blue-green algae (Cyanobacteria). These algae are abundant in surface water and do not generally persist in the absence of sunlight.

Insects/Larvae – This category includes insects and their larvae and eggs. Insect parts are not as significant as intact organisms since insects molt and the external skeleton could persist in water for long periods of time.

Golenkinia (Green Alga) & Cyclotella (Diatom)

Coelastrum (Green Alga)

Tribonema (Golden Alga)

Anabaena (Blue-Green Alga)

Insect Wing Scale

Rotifers – Organisms ranging in size 70-500 µm that are medium to good indicators of surface water influence, particularly when supported by the presence of other indicators.

Plant Debris – This category, in our opinion, is significant only when it relates to chlorophyll containing fragments of plant tissue, since the plant debris could persist in water for extended periods of time much beyond the viability of Giardia or other pathogen cysts.

Secondary Indicators

Nematodes – Nematodes and or their eggs are common in surface water and in ground waters with detritus and organic debris.

Crustaceans – Many species occur in surface waters.

Amoebae – Free living amoebae. Large numbers of amoebae in groundwater may indicate substantial bacterial populations or organic detritus in the water.

Non-photosynthetic flagellates and ciliates – Free-living protozoa are extremely common in healthy surface sources. Like amoebae, they feed on bacteria, algae, small metazoans, other protozoa and extraneous debris. Although many flagellates are photosynthetic, there are a number of species that grow in the absence of light providing sufficient dissolved nutrients are available.

Photosynthetic flagellates – Includes species such as Euglena. While these organisms are photosynthetic, many can persist in the dark for months and because they are motile, their presence may not be indicative of surface water contamination.

Other: Other organisms frequently seen in MPA samples include the iron bacteria. The presence of iron bacteria does not have significance for surface infiltration, but large numbers of iron bacteria can produce biofouling of the well.

The EPA risk factor tables are used to weight the results of MPA analyses. The greatest weight is given to the primary indicators.

Table 1 assigns a rating (Not Significant through Extremely Heavy).

Table 2 uses the ratings assigned by Table 1 to determine the Relative Risk Factor.

Table 1: Numerical range of each primary bio-indicator (particulate) counted per 100 gallons water.

Indicators of surface water1	EH3	H	M	R	NS
Giardia2	>30	16 to 30	6 to 15	1 to 5	<1
Coccidia2	>30	16 to 30	6 to 15	1 to 5	<1
Diatoms4	>150	41 to 149	11 to 40	1 to 10	<1
Other Algae4	>300	96 to 299	21 to 95	1 to 20	<1
Insects/Larvae	>100	31 to 99	16 to 30	1 to 15	<1
Rotifers	>150	61 to 149	21 to 60	1 to 20	<1
Plant Debris4	>200	71 to 200	26 to 70	1 to 25	<1

According to EPA “Guidance Manual for Compliance with the Filtration and Disinfection Requirements for Public Water Systems Using Surface Water Sources”, March, 1991
If Giardia cysts or coccidian are found in any sample, irrespective of volume, score as
Key: EH-Extremely High, H-Heavy, M-Moderate, R-Rare, NS-Not Significant
Chlorophyll Containing

Table 2: Relative surface water risk factors associated with scoring or primary bio-indicators (particulate) present during MPA of subsurface water sources.

Indicators of surface water1	Relative Risk Factor3
Indicators of surface water1	EH2	H	M	R	NS
Giardia	40	30	25	20	0
Coccidia	35	30	25	20	0
Diatoms	16	13	11	6	0
Other Algae	14	12	9	4	0
Insects/Larvae	9	7	5	3	0
Rotifers	4	3	2	1	0
Plant Debris	3	2	1	0	0

According to EPA “Guidance Manual for Compliance with the Filtration and Disinfection Requirements for Public Water Systems Using Surface Water Sources”, March, 1991
Key: EH – Extremely High, H – Heavy, M – Moderate, R – Rare, NS – Not Significant
Risk of surface water contamination:

>20 – High risk, 10-19 – Moderate risk, <9 – Low Risk

*Note: Any finding of Giardia or Cryptosporidium results in a minimum risk factor of 20.

*Appendix 1. Tables 1 & 2 from Vasconcelos, J. 1992 Consensus Method for Determining Groundwaters Under the Direct Influence of Surface Water Using Microscopic Particulates Analysis (MPA). U.S. Environmental Protection Agency, Region 10 Oct. p. 30-31.

A Brief Guide To Cryptosporidium & Giardia

Cyptosporidium is a microscopic protozoan parasite found in surface waters such as rivers and lakes that are used as drinking water supplies. A number of waterborne disease outbreaks in the U.S. and other countries have been attributed to Cryptosporidium, including a 1993 outbreak of over 400,000 cases in Milwaukee, Wisconsin.
Like Cryptosporidium, Giardia is a protozoan parasite found in surface waters. Giardia can cause gastrointestinal disease. Unlike Cryptosporidium infections, it is treatable with chemotherapy.
Interpretation of Cryptosporidium, Giardia positive results is difficult since the protozoans detected may be dead, the Cryptosporidium or Giardia detected may not be infective to humans, or the monitoring recoveries of the parasites are low. Only intact cysts or oocysts have the potential to be infective, however, the presence of even an empty Cryptosporidium or Giardia indicates infiltration into the water supply.
Environmental Associates Ltd. (EAL) offers testing for Cryptosporidium and Giardia by EPA Method 1623. With protozoan analysts surpassing national performance in recovery and identification of Cryptosporidium and Giardia during the ICR and LT2 performance evaluations, EAL is uniquely qualified for water source monitoring. Please call EAL to discuss your monitoring and source water assessment needs.

EPA LT2 Envirochek™ Field Filtration for Cryptosporidium – Appendix G

Appendix G

Envirochek™ Field Filtration for Cryptosporidium

Procedure for Field-Filtering Samples for Cryptosporidium Analysis Using the Pall Life Sciences Envirochek™ or Envirochek™ HV Capsules

1.0 Materials

The following materials should be available before collecting sample:

Several pairs of new, powder-free latex gloves
Sample collection form (Appendix F)
Pall Life Sciences Envirochek™ or Envirochek™ HV capsule (recommend that two capsules be kept on hand in case the first one clogs prior to filtering 10 L)
Sanitary $30 L carboy (if sample is not from a pressurized source)
Stopwatch
Sample label
Cooler, approximately 16-quart
Temperature monitoring device (e.g., thermometer vial, Thermochron™ iButton, or equivalent) (if measuring temperature during shipment)
Two large plastic trash bags
One 8-lb bag of ice or gel ice packs
Three gallon size ziplock bags
Strapping tape
Two self-adhesive plastic airbill sleeves
Airbill for shipment
The following items may be purchased as a unit for sampling from a vendor or laboratory supplying sampling apparatus for field filtering Cryptosporidium samples:
Pump (if sample is not from a pressurized source) – Electric centrifugal or Electric peristaltic pump, or any equivalent pump that can create a flow of approximately 2 L/min
Two 0.5-in. X 0.375-in. barbed reducing connectors
Five lengths of clean 12.7-mm (0.5-in.) internal-diameter clear, vinyl, laboratory tubing
Five pairs of hose clamps fit to tubing
One 0.5-in. x 3-in. nipple
One coupling to fit 0.5-in. internal diameter tubing
One roll teflon tape
One 0.5-in. x 0.5-in. x 0.25-in. tee Q Six 0.5-in. barbed male adapters One garden hose barbed adapter Pressure regulator

Q	Pressure gauge (capsule’s maximum operating pressure, Envirochek™ – 30 PSID, Envirochek™
	HV – 60 PSID)
Q	Water meter (flow totalizer)
Q	Flow rate meter with valve or flow control valve (capsule’s maximum flow rate, Envirochek™
	2 L/min, Envirochek™ HV – 4 L/min)
2.0	Collecting the Sample

If the sample will be collected from a pressurized source, use the sample collection procedures in Section

2.1. If the sample will be collected from an unpressurized source, use the sample collection procedures in Section 2.2.

2.1 Sample Filtration from a Pressurized Source

Before connecting the sampling system to the tap or source, turn on the tap and allow the water to flow for 2 to 3 minutes until the temperature has stabilized or until any debris that has accumulated in the source water lines has cleared and the turbidity in the water becomes visibly uniform. Turn off the

Put on a pair of powder-free latex gloves to prevent contamination from outside sources. Any contamination inside the sampling apparatus may bias the final

Determine the pressure of the water source with the pressure

Assemble the sampling system, minus the Envirochek™ /Envirochek™ HV capsule. In place of the Envirochek™ /Envirochek™ HV capsule, insert a 0.5-in. barbed connector between the outlet tubing from the sample valve or the pressure regulator or gauge and the inlet tubing of the flow totalizer, flow meter or control valve. For high pressure (>30 PSIG for Envirochek™ capsule or

>60 PSIG for Envirochek™ HV capsule) sites, the sampling system should be assembled in the following order, as shown in Figure 1 below:

Reinforced influent tubing
Pressure regulator
Pressure gauge
Reinforced inlet tubing
Envirochek™ /Envirochek™ HV capsule
Reinforced outlet tubing
Flow totalizer (mechanical or graduated collection device)
Flow rate meter with valve or flow control valve
Effluent tubing to drain

Figure 1.Sample System Setup for Collecting Cryptosporidium Samples from a Pressurized Source ( >30 PSIG for Envirochek™ capsule or >60 PSIG for Envirochek™ HV capsule)

For low pressure (1 to 30 PSIG for Envirochek™ capsule or 1 to 60 PSIG for Envirochek™ HV capsule) sites, the sampling system should be assembled at the sample port valve in the following order, as shown in Figure 2 below :

Influent tubing
Envirochek™ /Envirochek™ HV capsule
Outlet tubing
Flow rate meter with valve or flow control valve
Flow totalizer (mechanical or graduated collection device)
Effluent tubing to drain

Figure 2.Sample System Setup for Collecting Cryptosporidium Samples from a Pressurized Source (1 – 30 PSIG for Envirochek™ capsule or 1 – 60 PSIG for Envirochek™ HV capsule)

Connect the sampling system, with the connector in place of the Envirochek™ /Envirochek™ HV capsule, to the pressurized water system, using appropriate fittings and
Slowly turn the tap to fully open. Allow a minimum of 20 L to flush the system. During this period, perform the following steps:
Measuring the flow rate with the flow rate meter or the flow totalizer and a stopwatch, adjust the flow rate to approximately 2 L/min (approximately 0.5 gpm) for the Envirochek™ capsule or 4 L/min (approximately 1.0 gpm) for the Envirochek™ HV capsule. If a flow control valve at the appropriate flow rate is used, no adjustment of the flow rate is necessary. Using the pressure regulator, adjust the pressure to a maximum of 30 PSIG if using the Envirochek™ or 60 PSIG if using the Envirochek™ HV. Observe the system for leaks and take the necessary corrective action if any are
Record the following information on the sample collection form (Appendix F):
- Public water system (PWS) name
- PWS address
- Sampler name
- Sample ID (optional)
- Public Water System Identification (PWS ID) number
- Public Water System facility ID number
- Facility name
- Sample collection point ID O Sample collection point name Sample collection date
- Source water type (optional [but required for E. coli sample forms])
- Requested analysis (circle Cryptosporidium field sample for routine monitoring sample; circle both “Cryptosporidium field sample” and “Cryptosporidium matrix spike” sample if you are sending an additional sample with the monitoring sample for matrix spike analysis)
After the assembly has been flushed, measure the turbidity of the source water and any optional water quality parameters such as temperature, and/or

Turn off the water at the sample port valve when the flow rate has been adjusted and the system has been

Record the following information on the capsule label with a waterproof pen:

PWS ID

Facility name

Date of sample collection

Record the following information on the sample collection form:

Initial meter reading

Remove the connector and in its place, install the Envirochek™ /Envirochek™ HV capsule in line, securing the inlet and outlet ends with the appropriate fittings/clamps.

Note! Retain the vinyl caps provided with the Envirochek™ /Envirochek™ HV capsule. These caps will be needed to seal the capsule for shipment.

Slowly turn on the pressurized water source. Adjust the flow rate to approximately 2 L/min for Envirochek™ capsule or 4 L/min for Envirochek™ HV capsule, if necessary. Record the following information on the capsule label or sample collection form:

Start time

Vent the residual air in the capsule using the bleed valve by turning it counter-clockwise. When the capsule is full of water, close the bleed

Monitor the water meter. When the targeted volume (actual sample volumes will be selected by the utility, but volumes are typically 10 L [2.64 gal] to 50 L [13.2 gal] and preferred to be consistent between sampling events for each source) has passed through the Envirochek™

/Envirochek™ HV capsule, shut off the water source. Allow the pressure to decrease until the water stops.

Record the following information on the capsule label and/or sample collection form:

Stop time (when the water was shut off)

Final meter reading

Comments to laboratory, if needed

With the capsule inlet pointed up, loosen the outlet end of the Envirochek™ /Envirochek™ HV capsule and allow water to drain as much as possible. Water drainage from the capsule through the outlet is acceptable, as the sample has passed through the membrane. Opening the bleed valve during the draining will speed the process. Be sure to close the valve when

Disconnect the inlet end of the Envirochek™ /Envirochek™ HV capsule, making sure not to spill any of the water remaining in the capsule through the inlet port. This water is part of your sample. Capsule may be shipped with or without residual

Seal the inlet of the capsule with the vinyl end cap that was previously

Seal the outlet of the capsule with the vinyl end cap that was previously saved. Place the Envirochek™ /Envirochek™ HV capsule in a plastic ziplock bag for

Immediately following sample collection, place the bag containing the capsule in a refrigerator to chill prior to packing the shipping cooler for shipment. If no refrigerator is available, and the sample will not be shipped for several hours, place the bag in the shipping cooler with ice to chill. Replace the ice with fresh ice before

Note! Method 1622/1623 requires that the temperature of the sample upon arrival at the laboratory must be #20°C (but not frozen), and the laboratory must begin sample processing within 96 hours of sample collection. If the sample temperature and holding time requirements are not met, then the sample is invalid and must be recollected.

2.2 Sample Filtration Using an Unpressurized Source

Put on a pair of powder-free latex gloves to prevent contamination from outside sources. Any contamination inside the sampling apparatus may bias the final

If sampling from a source of unlimited volume, it may be desirable to pre-flush the sampling system. Assemble the sampling system, minus the Envirochek™ /Envirochek™ HV capsule. In place of the Envirochek™ /Envirochek™ HV capsule, insert a 0.5-in. coupling to connect the influent tubing to the inlet tubing of the pump. The sampling system should be assembled in the following order, as shown in Figure 3 below:

Influent tubing

Envirochek™ /Envirochek™ HV capsule

Outlet tubing

Centrifugal or peristaltic pump

Tubing

Flow rate meter with valve or flow control valve

Flow totalizer (mechanical or graduated collection device)

Effluent tubing to drain

Figure 3. Sample System Setup for Collecting Cryptosporidium Samples from an Unpressurized Source

When assembling sample chain, make sure that it is as airtight as possible in order to create a vacuum. To accomplish this, make sure that clamps are used at each connection and that rubber washers are inserted into the hose connections on the inlet and outlet ends of the centrifugal pump.

Place the inlet end of the inlet tubing into the sample source, away from any walls, bottom, or other environmental

Turn on the pump and allow a minimum of 20 L to flush the system. If sampling source water from a carboy, continuously refill the carboy as necessary to flush the system. As a recommendation, the carboy should not be removed from the sampling chain and should be refilled using a separate container. Observe the system for leaks and take the necessary corrective action if any are present. During this period, perform the following steps:

Measuring flow rate with the flow rate meter or the flow totalizer and a stopwatch, adjust the flow rate to approximately 2 L/min (approximately 0.5 gpm) for the Envirochek™ capsule or 4 L/min (approximately 1.0 gpm) for the Envirochek™ HV capsule by varying the pump speed or adjusting the valve (if pump is not variable speed). If a flow control valve at the appropriate flow rate is used, no adjustment of the flow rate is

Record the following information on the sample collection form:
- Public water system (PWS) name
- PWS address
- Sampler name
- Sample ID (optional)
- Public Water System Identification (PWS ID) number
- Public Water System facility ID number
- Facility name
- Sample collection point ID O Sample collection point name O Sample collection date
- Source water type (optional [but required for E. coli sample forms])
- Requested analysis (circle Cryptosporidium field sample for routine monitoring sample; circle both “Cryptosporidium field sample” and “Cryptosporidium matrix spike” sample if you are sending an additional sample with the monitoring sample for matrix spike analysis)

Turn off the pump when the flow rate has been adjusted and the assembly has been

Record the following information on the capsule label:

PWS ID

Facility name

Date of sample collection

Record the following information on the sample collection form:

Initial meter reading

Install the Envirochek™ /Envirochek™ HV capsule in line, securing the inlet and outlet ends with the appropriate fittings/clamps.

Note! Retain the vinyl caps provided with the Envirochek™ /Envirochek™ HV capsule. These caps will be needed to seal the capsule for shipment.

Refill the carboy with the sample to be filtered. As a recommendation, the carboy should not be removed from the sampling assembly and should be refilled using a separate

Turn on the pump. Adjust the flow rate to approximately 2 L/min for the Envirochek™ capsule or 4 L/min for the Envirochek™ HV capsule, if necessary. Record the following information on the capsule label and/or sample collection form:

Start time

Monitor the water meter continuously refilling the carboy as needed. When the targeted volume (actual sample volumes are selected by the utility, but volumes are typically 10 L [2.64 gal] to 50 L [13.2 gal] and preferred to be consistent between sampling events for each source) is reached, remove tubing from the carboy and then shut off the

Record the following information on the capsule label and/or sample collection form:

Stop time (when the pump was shut off)

Final meter reading

Comments to laboratory, if needed

With the capsule inlet pointed up, loosen the outlet end of the Envirochek™ /Envirochek™ HV capsule and allow water to drain as much as possible. Water drainage from the capsule through the outlet is acceptable, as the sample has passed through the membrane. Opening the bleed valve during the draining will speed the process. Be sure to close the valve when

Disconnect the inlet end of the Envirochek™ /Envirochek™ HV capsule, making sure not to spill any of the water remaining in the capsule through the inlet port. This water is part of your sample. Capsule may be shipped with or without residual

Seal the inlet of the capsule with the vinyl end cap that was saved

Seal the outlet of the capsule with the vinyl end cap that was saved previously. Place the Envirochek™ /Envirochek™ HV capsule in a plastic ziplock bag for

Immediately following sample collection, place the bag containing the capsule in a refrigerator to chill prior to packing the shipping cooler for shipment. If no refrigerator is available, and the sample will not be shipped for several hours, place the bag in the shipping cooler with ice to chill. Replace the ice before

3.0 Packing the Sample

Insert two large plastic trash bags into the shipping cooler to create a double liner. Immediately before packing the cooler, create two 4-pound bags of ice in two separate ziplock bags. To prevent leaks place each ice pack into an additional ziplock bag. Gel packs or blue ice may be used instead of wet ice, as long as the sample is maintained in the appropriate temperature Seal the ziplock bags, expelling as much air as possible, and secure top with tape.

Note! Shipping companies may delay sample shipments if leakage occurs. Double liners and ziplock bags around ice will prevent leakage and delays.

Place the bag containing the capsule into the shipping container. Wrap the capsule in bubble wrap to prevent freezing. Inflated, empty sample bags can be placed between the capsule and the ice packs to prevent the sample from

If you will be monitoring sample temperature during shipment, place in the cooler the temperature monitoring device (e.g., extra sample bottle for measuring sample temperature upon receipt at the laboratory, thermometer vial, or Thermochron™ iButton). Seal each liner bag by twisting top of bag and tying in a

Peel the backing off one of the plastic airbill sleeves and attach the sleeve to the inside of the cooler

Sign and date the sample collection form.

Fold the completed sample collection form, and place it inside the plastic sleeve.

Close the cooler lid, seal the horizontal joints with strapping tape, and secure the lid with tape by taping the cooler at each end, perpendicular to the

Note! Shipping companies may delay sample shipments if leakage occurs. Be sure to seal the cooler joints.

Peel the backing off of the second airbill sleeve and attach the sleeve to the outside of the cooler lid. Complete the shipping airbill with the laboratory address, billing information, sample weight, and shipping service. Remove the shipper’s copy of the airbill, and place the remainder of the airbill inside the plastic

4.0 Shipping and Tracking

Ship samples on the day of collection and use a reliable shipping service for overnight delivery. If samples are not shipped the day of collection, the sample must be maintained between 1°C and 10°C (but not frozen) by chilling in a refrigerator or cooler filled with

Contact the laboratory to notify them of the sample shipment. Request that the laboratory contact you the next day if the sample is not

Using the airbill number on the shipper’s copy of the airbill, track the sample shipment using the shipping company’s web page or by contacting the shipping company over the

If problems are encountered with the shipment, communicate with the shipping company to resolve, and update the laboratory regarding the status of the

EPA Fact Sheet – LT2 Cryptosporidium – Long Term 2 Enhanced Surface Water Treatment Rule

Original PDF from EPA

In the past 30 years, the Safe Drinking Water Act (SDWA) has been highly effective in protecting public health and has also evolved to respond to new and emerging threats to safe drinking water. Disinfection of drinking water is one of the major public health advances in the 20th century. One hundred years ago, typhoid and cholera epidemics were common through American cities; disinfection was a major factor in reducing these epidemics.

In the past 15 years, we have learned that there are specific microbial pathogens, such as Cryptosporidium, which can cause illness, and are highly resistant to traditional disinfection practices. We also know that the disinfectants themselves can react with naturally-occurring materials in the water to form byproducts, which may pose health risks.

Amendments to the SDWA in 1996 require EPA to develop rules to balance the risks between microbial pathogens and disinfection byproducts (DBPs). The Stage 1 Disinfectants and Disinfection Byproducts Rule and Interim Enhanced Surface Water Treatment Rule, promulgated in December 1998, were the first phase in a rulemaking strategy required by Congress as part of the 1996 Amendments to the Safe Drinking Water Act.

The Long Term 2 Enhanced Surface Water Treatment Rule builds upon earlier rules to address higher risk public water systems for protection measures beyond those required for existing regulations.

The Long Term 2 Enhanced Surface Water Treatment Rule and the Stage 2 Disinfection Byproduct Rule are the second phase of rules required by Congress. These rules strengthen protection against microbial contaminants, especially Cryptosporidium, and at the same time, reduce potential health risks of DBPs.

Questions and Answers

What is the LT2ESWTR?

The purpose of Long Term 2 Enhanced Surface Water Treatment Rule (LT2ESWTR) is to reduce illness linked with the contaminant Cryptosporidium and other pathogenic microorganisms in drinking water. The LT2ESWTR will supplement existing regulations by targeting additional Cryptosporidium treatment requirements to higher risk systems. This rule also contains provisions to reduce risks from uncovered finished water reservoirs and provisions to ensure that systems maintain microbial protection when they take steps to decrease the formation of disinfection byproducts that result from chemical water treatment.

Current regulations require filtered water systems to reduce source water Cryptosporidium levels by 2-log (99 percent). Recent data on Cryptosporidium infectivity and occurrence indicate that this treatment requirement is sufficient for most systems, but additional treatment is necessary for certain

higher risk systems. These higher risk systems include filtered water systems with high levels of Cryptosporidium in their water sources and all unfiltered water systems, which do not treat for

Cryptosporidium.

The LT2ESWTR is being promulgated simultaneously with the Stage 2 Disinfection Byproduct Rule to address concerns about risk tradeoffs between pathogens and DBPs.

What are the health risks of Cryptosporidium?

Cryptosporidium is a significant concern in drinking water because it contaminates most surface waters used as drinking water sources, it is resistant to chlorine and other disinfectants, and it has caused waterborne disease outbreaks. Consuming water with Cryptosporidium can cause gastrointestinal illness, which may be severe and sometimes fatal for people with weakened immune systems (which may include infants, the elderly, and people who have AIDS).

Who must comply with this rule?

This regulation will apply to all public water systems that use surface water or ground water under the direct influence of surface water.

What does the rule require?

Monitoring: Under the LT2ESWTR, systems will monitor their water sources to determine treatment requirements. This monitoring includes an initial two years of monthly sampling for Cryptosporidium. To reduce monitoring costs, small filtered water systems will first monitor for E. coliCa bacterium which is less expensive to analyze than CryptosporidiumCand will monitor for Cryptosporidium only if their E. coli results exceed specified concentration levels.

Monitoring starting dates are staggered by system size, with smaller systems beginning monitoring after larger systems. Systems must conduct a second round of monitoring six years after completing the initial round to determine if source water conditions have changed significantly. Systems may use (grandfather) previously collected data in lieu of conducting new monitoring, and systems are not required to monitor if they provide the maximum level of treatment required under the rule.

Cryptosporidium treatment: Filtered water systems will be classified in one of four treatment categories (bins) based on their monitoring results. The majority of systems will be classified in the lowest treatment bin, which carries no additional treatment requirements. Systems classified in higher treatment bins must provide 90 to 99.7 percent (1.0 to 2.5-log) additional treatment for Cryptosporidium. Systems will select from a wide range of treatment and management strategies in the Amicrobial toolbox@ to meet their additional treatment requirements. All unfiltered water systems must provide at least 99 or 99.9 percent (2 or 3-log) inactivation of Cryptosporidium, depending on the results of their monitoring. These Cryptosporidium treatment requirements reflect consensus recommendations of the Stage 2 Microbial and Disinfection Byproducts Federal Advisory Committee.

Other requirements: Systems that store treated water in open reservoirs must either cover the reservoir or treat the reservoir discharge to inactivate 4-log virus, 3-log Giardia lamblia, and 2-log Cryptosporidium. These requirements are necessary to protect against the contamination of water that occurs in open reservoirs. In addition, systems must review their current level of microbial treatment before making a significant change in their disinfection practice. This review will assist systems in maintaining protection against microbial pathogens as they take steps to reduce the formation of disinfection byproducts under the Stage 2 Disinfection Byproducts Rule, which EPA is finalizing along with the LT2ESWTR.

What are the benefits of the rule?

The LT2ESWTR will improve the control of Cryptosporidium and other microbiological pathogens in drinking water water systems with the highest risk levels. EPA estimates that full compliance with the LT2ESWTR will reduce the incidence of cryptosporidiosis – the gastrointestinal illness caused by ingestion of Cryptosporidium – by 89,000 to 1,459,000 cases per year, with an associated reduction of 20 to 314 premature deaths. The monetized benefits associated with these reductions ranges from $253 million to $1.445 billion per year. The additional Cryptosporidium treatment requirements of the LT2ESWTR will also reduce exposure to other microbial pathogens, such as Giardia, that co-occur with Cryptosporidium. Additional protection from microbial pathogens will come from provisions in this rule for reviewing disinfection practices and for covering or treating uncovered finished water reservoirs, though EPA has not quantified these benefits.

What are the costs of the rule?

The LT2ESWTR will result in increased costs to public water systems and states. The average annualized present value costs of the LT2ESWTR are estimated to range from $92 to $133 million (using a three percent discount rate). Public water systems will bear approximately 99 percent of this total cost, with states incurring the remaining 1 percent. The average annual household cost is estimated to be $1.67 to $2.59 per year, with 96 to 98 percent of households experiencing annual costs of less than $12 per year.

What technical information will be available on the rule?

The following guidance documents will be available:

• Source Water Monitoring Guidance

• Microbial Laboratory Guidance

• Small Entity Compliance Guidance

• Microbial Toolbox Guidance Manual

• Ultraviolet Disinfection Guidance Manual

• Membrane Filtration Guidance Manual

• Simultaneous Compliance Guidance Manual

• Low-pressure Membrane Filtration for Pathogen Removal: Application, Implementation, and Regulatory Issues

Where can I find more information about this notice and the LT2ESWTR?

For general information on the LT2ESWTR, contact the Safe Drinking Water Hotline at (800) 426-4791. The Safe Drinking Water Hotline is open Monday through Friday, excluding legal holidays, from 10:00 a.m. to 4:00 p.m., Eastern time. For copies of the Federal Register notice of the regulation or technical fact sheets, visit the EPA Safewater website at http://www.epa.gov/safewater/disinfection/lt2. For technical inquiries, email stage2mdbp@epa.gov.

Office of Water (4607M) EPA 815-F-05-009 December 2005 www.epa.gov/safewater

Good News for Ohio LT2 EPA Method 1623 Cryptosporidium and E. coli Clients

Environmental Associates Ltd. will fill your LT2 Method 1623 & E.Coli paperwork out for Ohio. If you use http://eal-labs.com/submit/ and sign the form by mouse, tablet or smart phone, we will then send you a copy of the filled out Ohio form to you with your final report. I think we all can stand to deal with less paperwork in our lives.

Cheers!!

Ohio EPA - LT2 Sample Data Entry Form — Ohio EPA – LT2 ESWTR Cryptosporidium Sample Collection Form

Ohio EPA - LT2 Sample Ecoli and Turbidity Data Entry Form — Ohio EPALT2 ESWTR E. coli and Turbidity Sample Collection Form